/

Biologia do Desenvolvimento

/

斑马鱼胚胎卵黄细胞中的 AFM 和 Microrheology

JoVE Journal
Biologia do Desenvolvimento

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia do Desenvolvimento

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Please note that all translations are automatically generated. Click here for the English version.

斑马鱼胚胎卵黄细胞中的 AFM 和 Microrheology

DOI:

10.3791/56224-v

•

09:47 min

•

November 29, 2017

•

Maria Marsal, Ignasi Jorba, Elena Rebollo, Tomas Luque, Daniel Navajas, Enrique Martín-Blanco

¹Instituto de Biología Molecular de Barcelona,Consejo Superior de Investigaciones Científicas, ²Institute for Bioengineering of Catalonia,Universitat de Barcelona and CIBER Enfermedades Respiratorias

Capítulos

00:05Título
01:08Atomic Force Microscopy
03:54Particle Tracking Microrheology
07:02Results: Cortical Tension Measurements and Rheology of the Cortex and Yolk of the Zebrafish Embryo Yolk Cell During Epiboly
08:46Conclusion

Summary

Tadução automática

缺少用于测量材料属性和拉伸参数的工具在体内可防止在开发过程中验证它们的角色。我们使用原子力显微镜 (AFM) 和纳米粒子跟踪来量化外包期间完整的斑马鱼胚胎卵黄细胞的机械特性。这些方法是可靠的, 广泛适用于避免介入干预。

Tags

AFM Microrheology Zebrafish Embryo Yolk Cell Mechanical Properties Stiffness Viscosity Adhesion Mechanobiology Embryo Mounting Embryo Handling Agarose Low Melting Agarose Inverted Optical Microscope Atomic Force Microscope

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

微珠植入斑马鱼胚胎

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

在活斑马鱼胚胎成像的亚细胞结构

在GFP-转基因斑马鱼使用Photoconvertible PSmOrange系统追踪单元

日本鹌鹑胚胎的卵黄囊膜主内胚上皮细胞培养

观察有丝分裂和动力学在现场斑马鱼胚胎

通过流式细胞术鉴定小鼠胚胎中的红细胞生成素及其后代

利用 Immunolabeling 分析斑马鱼胚胎中的稳定、动态和初生的微管

在斑马鱼胚胎中通过细胞 Photoconversion 的心内膜组织运动后

家兔模型最佳胚胎阶段微创胚胎移植与胚胎玻璃化

Read Article