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腺相关病毒和共聚焦显微镜对器官脑切片胶质细胞器的可视化和实时成像

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

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腺相关病毒和共聚焦显微镜对器官脑切片胶质细胞器的可视化和实时成像

DOI:

10.3791/56237-v

•

08:38 min

•

October 23, 2017

•

Lauritz Hagen Kennedy, Johanne Egge Rinholm²

¹Division of Anatomy, Institute of Basic Medical Sciences,University of Oslo, ²Department of Microbiology,Oslo University Hospital

Capítulos

00:05Título
00:46Viral Transduction
01:52Microscope Set-up
03:24Time-lapse Imaging
06:52Results: Time-lapse Imaging to Track Mitochondrial Movement in Oligodendrocytes
08:12Conclusion

Summary

Tadução automática

形成突促进了快速动作电位的传播和神经元的存活。这里描述的是一个协议的胶质特异表达荧光蛋白在器官脑切片与后续的延时成像。此外, 一个简单的程序, 可视化不髓鞘提出。

Tags

Oligodendrocyte Organelle Organotypic Brain Slice Adeno-associated Virus Confocal Microscopy Mitochondria Myelin Time-lapse Imaging Viral Transduction

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