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Oprichting van larvale zebravis als Model van een dier te onderzoeken Trypanosoma cruzi Motility In Vivo
JoVE Journal
Biologia do Desenvolvimento
Author Produced
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo
DOI:

13:21 min

September 30, 2017

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Capítulos

  • 00:00Título
  • 00:13Introduction
  • 03:09Obtaining Embryos for Injection
  • 04:13Preparing Injection Material
  • 05:38Obtaining Labeled T. cruzi Parasites for Injection
  • 07:04Injecting Zebrafish Larvae
  • 08:10LSFM Mounting and Imaging of Injected Larvae
  • 08:57LSFM Imaging of Injected Larvae
  • 10:44Representative Results
  • 11:40Conclusion

Summary

Tadução automática

In dit protocol, fluorescently geëtiketteerde T. cruzi werden ingespoten in transparante zebrafish larven en de beweeglijkheid van de parasiet werd waargenomen in vivo met licht blad fluorescentie microscopie.

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