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Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
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Capítulos
- 00:05Título
- 00:38siRNA Transient HIV-Tat-interactive Protein 60 kDa (TIP60) Depletion
- 02:08Wound-healing Assay Cell Seeding and TIP60 Knockdown Efficiency Evaluation
- 04:10Wound Creation, Imaging, and Single Cell Movement Tracking
- 06:32Results: Representative Videos and Quantifications of siTIP60 and siControl Cell Migration
- 07:49Conclusion
Here, we present the real-time monitoring of cell migration in a wound-healing assay using TIP60-depleted MCF10A breast epithelial cells. The implementation of live-cell imaging techniques in our protocol allows us to analyze and visualize single-cell movement in real time and across time.
