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TIP60 短暂耗竭后乳腺上皮细胞迁移的活体影像学研究

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Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

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TIP60 短暂耗竭后乳腺上皮细胞迁移的活体影像学研究

DOI:

10.3791/56248-v

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08:13 min

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December 07, 2017

•

Yanzhou Zhang, Grace SuShin Chia, Cheng Yong Tham, Sudhakar Jha²

¹Cancer Science Institute of Singapore,National University of Singapore, ²Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore

Capítulos

00:05Título
00:38siRNA Transient HIV-Tat-interactive Protein 60 kDa (TIP60) Depletion
02:08Wound-healing Assay Cell Seeding and TIP60 Knockdown Efficiency Evaluation
04:10Wound Creation, Imaging, and Single Cell Movement Tracking
06:32Results: Representative Videos and Quantifications of siTIP60 and siControl Cell Migration
07:49Conclusion

Summary

Tadução automática

在这里, 我们提出了实时监测细胞迁移在伤口愈合试验中使用 TIP60-depleted MCF10A 乳腺上皮细胞。在我们的协议中实现了活细胞成像技术, 使我们能够实时和跨时间地分析和可视化单细胞运动。

Tags

Keywords: Live-imaging Breast Epithelial Cell Cell Migration TIP60 Depletion Cancer Metastasis Cell Morphology SIRNA Transfection Cell Culture Cell Counting Cell Seeding Gene Knockdown Efficiency

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