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流感血凝素特异抗体诱导的 Fc 介导效应函数的一种评估方法

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

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流感血凝素特异抗体诱导的 Fc 介导效应函数的一种评估方法

DOI:

10.3791/56256-v

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04:47 min

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February 23, 2018

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Mark J. Bailey², Felix Broecker, Paul E. Leon², Gene S. Tan

¹Department of Microbiology,Icahn School of Medicine at Mount Sinai, ²Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, ³Department of Infectious Disease,J. Craig Venter Institute

Capítulos

00:05Título
00:42Influenza Virus Hemagglutinin Expression
02:18Antibody-dependent Cell-mediated Cytotoxicity (ADCC) Analysis
04:01Results: Representative ADCC Analysis
04:24Conclusion

Summary

Tadução automática

我们描述了一种方法来衡量 Fc 介导的效应函数的活化作用的抗体, 针对流感病毒血凝素。这种检测也可以适应评估单克隆抗体或多克隆血清靶向其他病毒表面糖蛋白, 以诱导 Fc 介导的免疫能力。

Tags

Keywords: Fc-mediated Effector Functions Influenza Hemagglutinin Antibody ADCC Cell-mediated Cytotoxicity Transfection Infection A549 Cells 293 Cells Virus Immunology Vaccinology

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