Journal
/
Neurociência
/
Desensitization and Recovery of Crayfish Photoreceptors Upon Delivery of a Light Stimulus
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Desensitization and Recovery of Crayfish Photoreceptors Upon Delivery of a Light Stimulus

Desensitization and Recovery of Crayfish Photoreceptors Upon Delivery of a Light Stimulus

DOI:
10.3791/56258-v

•

06:43 min

•

November 09, 2019

•

, , , , ,
1Departamento de Fisiología, Facultad de Medicina,Universidad Nacional Autónoma de México, 2Laboratorio Nacional de Canalopatías, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Capítulos

  • 00:04Título
  • 01:01Experimental Setup
  • 02:40Eyestalk Isolation and Photoreceptor Impaling
  • 04:01Electrical Recording
  • 04:56Results: Recovery from Desensitization
  • 05:42Conclusion

Summary

Tadução automática

Tadução automática

A protocol for the study of desensitization and sensitivity recovery of crayfish photoreceptors as a function of circadian time is presented.

Tags

Crayfish PhotoreceptorsDesensitizationSensitivity RecoveryCircadian TimeIntracellular Electrical RecordingsSingle Electrode Switched Voltage ClampNeurobiologyPhotoreceptor Electrical ActivityCell IsolationIntracellular MilieuMicropipette PullerPotassium Chloride SolutionElectrode ResistanceBridge ModeOffset CurrentCapacitive TransientsSuperfusion SystemEye Stock Isolation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/56258?article_type=v

Recording Behavioral Responses to Reflection in Crayfish
check_url/pt/56258?article_type=v

Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle
check_url/pt/56258?article_type=v

Muscle Receptor Organs in the Crayfish Abdomen: A Student Laboratory Exercise in Proprioception
check_url/pt/56258?article_type=v

Physiological Experimentation with the Crayfish Hindgut: A Student Laboratory Exercise
check_url/pt/56258?article_type=v

Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity
check_url/pt/56258?article_type=v

Behavioral Determination of Stimulus Pair Discrimination of Auditory Acoustic and Electrical Stimuli Using a Classical Conditioning and Heart-rate Approach
check_url/pt/56258?article_type=v

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear
check_url/pt/56258?article_type=v

The Swimmeret System of Crayfish: A Practical Guide for the Dissection of the Nerve Cord and Extracellular Recordings of the Motor Pattern
check_url/pt/56258?article_type=v

Electrophysiological Method for Recording Intracellular Voltage Responses of Drosophila Photoreceptors and Interneurons to Light Stimuli In Vivo
check_url/pt/56258?article_type=v

Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light

Read Article