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一种基于猪脑内皮细胞的体外血脑屏障模型的改进方法
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JoVE Journal Medicina
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
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一种基于猪脑内皮细胞的体外血脑屏障模型的改进方法

DOI:
10.3791/56277-v

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09:23 min

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September 24, 2017

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, , , , , , ,
1Lundbeck Foundation Research Initiative on Brain Barriers and Drug Delivery, Department of Biomedicine,Aarhus University, 2Institute of Pharmaceutical Science,King’s College London, 3HICoE Centre for Drug Research,Universiti Sains Malaysia

Capítulos

  • 00:05Título
  • 00:44Purification of Porcine Brain Capillaries
  • 03:14Cultivation of Primary pBECs
  • 06:53TEER Measurements
  • 07:47Results: Representative Time Course of Initial Cultures of pBECs and Rat Astrocytes
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

该协议的目的是提出一个优化的程序, 建立一个体外血脑屏障 (BBB) 模型的基础上猪脑内皮细胞 (pBECs)。该模型具有重现性高、严密性高的优点, 适合于研究药物发现中的转运和胞内贩运。

Tags

Blood-brain BarrierPorcine Brain Endothelial CellsIn Vitro ModelPrimary Cell IsolationCapillary IsolationCell DigestionCell CultureTEERPermeability

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