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角膜组织工程: 人角膜间质神经相互作用的体外模型

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

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角膜组织工程: 人角膜间质神经相互作用的体外模型

DOI:

10.3791/56308-v

•

07:35 min

•

January 24, 2018

•

Rabab Sharif, Shrestha Priyadarsini, Tyler G. Rowsey, Jian-Xing Ma, Dimitrios Karamichos²

¹Department of Cell Biology,University of Oklahoma Health Sciences Center, ²Department of Ophthalmology/Dean McGee Eye Institute,University of Oklahoma Health Sciences Center, ³Department of Physiology, Harold Hamm Diabetes Center,University of Oklahoma Health Sciences Center

Capítulos

00:05Título
01:17Isolation of Primary Cells
04:04Primary HCF Culture and 3D Construct Assembly
05:00Cell Differentiation and Co-culture
06:17Results: Transmission Electron Microscopy of the 3D Self-assembled Construct
06:48Conclusion

Summary

Tadução automática

本议定书描述了一个新的三维的体外模型, 其中角膜基质细胞和分化的神经细胞被培养在一起, 以协助检查和理解两种细胞的相互作用。

Tags

Stromal-nerve Interactions Human Cornea Corneal Diseases Corneal Dystrophies Extracellular Matrix Cell-cell Interactions Drug Screening Corneal Nerve Damage Corneal Nerve Regeneration

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