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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

DOI:
10.3791/56312-v

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07:38 min

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September 25, 2017

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, ,
1Department of Pharmacology,University of Minnesota, 2Masonic Cancer Center,University of Minnesota

Capítulos

  • 00:05Título
  • 00:46Preparation of Nitrosamide Positive Control
  • 03:07Nitrosamine-P450 In Vitro Incubation
  • 04:58Results: Nitrosamide Standard Preparation and In Vitro Incubation
  • 06:19Conclusion

Summary

Tadução automática

Tadução automática

α-hydroxylation of carcinogenic nitrosamines by cytochrome P450s is the accepted metabolic pathway that produces DNA-damaging intermediates, which cause mutations. However, new data indicates further oxidation to nitrosamides can occur. We describe a general method for detecting nitrosamides produced from in vitro cytochrome P450-catalyzed metabolism of nitrosamines.

Tags

NitrosamideNitrosamineCytochrome P450In Vitro MetabolismMass SpectrometryCarcinogenesisNorcotinineAcetic AnhydrideSodium NitriteColumn ChromatographyNMRBaculosomesNADPH Regeneration System

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