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线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究

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Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro

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线粒体膜电位检测 CLIC4 击倒诱导 HN4 细胞凋亡的实验研究

DOI:

10.3791/56317-v

•

13:20 min

•

July 17, 2018

•

Jinsen Lu, Lele Wu, Xiaoke Wang, Jinhang Zhu, Juan Du, Bing Shen

¹School of Basic Medical Sciences,Anhui Medical University, ²Department of Orthopedics, Anhui Provincial Hospital,Anhui Medical University

Capítulos

00:04Título
00:33Cell Transfection
02:08Rh123 Labeling
04:21Detection of the Mitochondrial Membrane Potential (MMP)
06:29Confocal Laser Scanning Fluorescence Microscope
08:34Statistical Analysis
09:48Results: Detection of Mitochondrial Membrane Potential via Normal Fluorescent Microscopy and Confocal Laser Scanning Microscopy
11:53Conclusion

Summary

Tadução automática

在这里, 我们提出了一个详细的协议, 应用罗丹明123识别线粒体膜电位 (基质) 和研究 CLIC4 击倒诱导 HN4 细胞凋亡的体外。在普通荧光显微镜和共聚焦激光扫描荧光显微镜下, 记录了基质金属蛋白酶的实时变化。

Tags

Keywords: Mitochondria Membrane Potential CLIC4 Knockdown HN4 Cell Apoptosis Rhodamine-123 Polylysine Trypsin Cell Culture Transfection SiRNA

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