É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Department of Translational Research,Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research),Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University
Capítulos
- 00:05Título
- 01:14Genomic DNA Extractions from S. mutans
- 04:01PCR Amplification
- 06:43Cell Transformation
- 07:43Verification of Gene Disruption
- 08:41Phenotypic Analysis
- 10:16Results: 2-Step Fusion PCR Generates S. mutans Strains with Gene Disruptions
- 11:26Conclusion
