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无细胞生化荧光酶法测定高密度脂蛋白脂质过氧化的高通量测定

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

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无细胞生化荧光酶法测定高密度脂蛋白脂质过氧化的高通量测定

DOI:

10.3791/56325-v

•

07:29 min

•

October 12, 2017

•

Shubhendu Sen Roy, Huy Cong Xuan Nguyen, Thomas A. Angelovich³, Anna C. Hearps, Diana Huynh⁴, Anthony Jaworowski⁴, Theodoros Kelesidis

¹University of California, Los Angeles, ²Centre for Biomedical Research,Burnet Institute, ³School of Health and Biomedical Sciences,RMIT University, ⁴Department of Infectious Diseases,Monash University

Capítulos

00:05Título
00:58Day 1: Preparation of Controls and Laboratory-specific Quality Controls
02:10Day 1: Separation of HDL Cholesterol using HDL Precipitation
03:07Day 2: Fluorochrome Assay
05:17Results: Cell-free Biochemical Fluorometric Assay Results
06:32Conclusion

Summary

Tadução automática

我们在这里描述了一种荧光无细胞生化测定高密度脂蛋白脂质过氧化的方法。这种快速和重现性的分析可以用来确定高密度脂蛋白功能的大规模研究, 并能有助于我们的理解 hdl 功能的人类疾病。

Tags

Keywords: Cell-free Biochemical Fluorometric Enzymatic Assay High-throughput Lipid Peroxidation High Density Lipoprotein HDL Function Cardiovascular Disease Metabolic Abnormalities Inflammatory Diseases HDL-C HDLox Pooled Quality Control HDL Cholesterol Precipitating Reagent HRP Fluorochrome Reagent DMSO

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