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MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

DOI:
10.3791/56326-v

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08:14 min

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October 26, 2017

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, , , , , , , ,
1Department of Experimental Oncology and Molecular Medicine,Fondazione IRCCS Istituto Nazionale dei Tumori, 2Unit of Thoracic Surgery,Fondazione IRCCS Istituto Nazionale dei Tumori

Capítulos

  • 00:05Título
  • 01:12Plasma Sample Collection and RNA Extraction
  • 03:20Taq-based Reverse Transcription and Pre-amplification
  • 04:34RT-qPCR Reaction
  • 05:46Results: Quality Control of Plasma Samples and RT-qPCR Performance Evaluation
  • 07:15Conclusion

Summary

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Tadução automática

Here, we present the detailed protocol adopted in the BioMILD screening trial to perform the circulating microRNA signature classifier test for early lung cancer detection.

Tags

Liquid BiopsyPlasma MiRNALung Cancer ScreeningMicroRNA SignatureEarly DetectionTumor MicroenvironmentRNA IsolationQPCRMolecular Analysis

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