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One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
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Capítulos
- 00:05Título
- 01:31Cell Culture and Irradiation
- 02:53Fixation and DAPI Staining
- 04:44Results: Nuclear Morpholgy in Different Forms of Cell Death
- 05:51Conclusion
Research on ionizing radiation (IR)-induced clonogenic cell death is important for understanding the effects of IR on malignant tumors and normal tissues. Here, we describe a one-step assay for assessing the major modes of IR-induced clonogenic cell death based on morphology of 4',6-diamidino-2-phenylindole dihydrochloride (DAPI)-stained nuclei visualized by fluorescence microscopy.
