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小鼠胚胎干细胞向皮质 Interneuron 前体细胞的分化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

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小鼠胚胎干细胞向皮质 Interneuron 前体细胞的分化

DOI:

10.3791/56358-v

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10:24 min

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December 03, 2017

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David J. Tischfield², Stewart A. Anderson

¹Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania, ²Department of Psychiatry, Children’s Hospital of Philadelphia,University of Pennsylvania School of Medicine

Capítulos

00:05Título
00:44Culturing mESCs
02:14Differentiation mESCs Toward MGE-like Telencephalic Progenitors
04:57Somatostatin-enriching Protocol: “DD12 GFP High Sonic Hedgehog (SHH)”
06:36PV-enriching Protocol: “DD11/DD17 mCherry + aPKCi”
07:53Generation of Nkx2.1::mCherry and Lhx6::GFP Interneuron Precursors
09:11Conclusion

Summary

Tadução automática

本议定书描述了一种方法, 产生皮层 interneuron 祖细胞和后有丝分裂 interneuron 前体从小鼠胚胎干细胞使用改良的胚体-单层法。这些祖/前体可用于体外或荧光分类和移植到新生儿大脑皮层研究命运确定, 或用于治疗应用。

Tags

Mouse Embryonic Stem Cells Cortical Interneuron Progenitors Nkx2.1 Parvalbumin Somatostatin MEF Feeder Cells Trypsin-EDTA Gelatin-coated Plates KSR-N2 Media Embryoid Bodies

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