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Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia
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JoVE Journal Medicina
Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking (TXL) for Myopia

DOI:
10.3791/56385-v

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12:25 min

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January 06, 2018

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, , ,
1Department of Ophthalmology,Columbia University College of Physicians and Surgeons, 2Confocal and Specialized Microscopy Shared Resource, Herbert Irving Comprehensive Cancer Center,Columbia University

Capítulos

  • 00:05Título
  • 01:10Tissue Preparation
  • 02:25SHG Imaging (Microscopy Protocol)
  • 08:27DSC Protocol
  • 09:26Results: Changes in Sclera after Cross-linking with Sub-Tenon’s Injection of Sodium Hydroxymethylglycinate
  • 10:52Conclusion

Summary

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This protocol describes techniques for evaluating chemical cross-linking of the rabbit sclera using second harmonic generation imaging and differential scanning calorimetry.

Tags

Keywords: Second Harmonic GenerationSHGMicroscopyScleraTXLMyopiaCollagenSub-tenon’s InjectionFormaldehydeDifferential Scanning CalorimetryNon-invasiveRabbit EyeEyelid SpeculumConjunctivaExtra Ocular MusclesScleral InsertionPBSObjective LensNumerical ApertureImmersion Gel

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