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1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry,University of Washington
Capítulos
- 00:04Título
- 01:17Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
- 04:31Screening the Library for Reactivation Using Hygromycin B Selection
- 06:44Validating the Identified Hairpins ( Hits )
- 09:27Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
- 10:17Conclusion
हम एक छोटी सी hairpin आरएनए (shRNA) और अगली पीढ़ी के अनुक्रमण-आधारित प्रोटोकॉल की पहचान के लिए एक murine सेल लाइन में एक्स गुणसूत्र निष्क्रियण के साथ-जुगनू luciferase और hygromycin प्रतिरोध के साथ जुड़े जीन मिथाइल CpG बाध्यकारी प्रोटीन 2 ( MeCP2) निष्क्रिय एक्स गुणसूत्र पर जीन ।
