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JoVE Journal Genetics
Poolade shRNA skärm för reaktivering av MeCP2 på inaktiva X-kromosomen
 
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Poolade shRNA skärm för reaktivering av MeCP2 på inaktiva X-kromosomen

Article DOI: 10.3791/56398-v • 11:15 min • March 2nd, 2018
March 2nd, 2018 •
1,2, 1, 1, 1, 1, 1, 1, 3, 1,4
1Clinical Research Division, Fred Hutchinson Cancer Research Center, 2Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 3Epigenetics Program, Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 4Departments of Medicine and Biochemistry, University of Washington

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Vi rapporterar en liten hårnål RNA (shRNA) och nästa generations sekvensering-baserat protokoll för att identifiera regulatorer av x-kromosomen inaktivering i en murin cellinje med firefly luciferas och hygromycin antibiotikaresistensgener smält till metyl CpG bindande protein 2) MeCP2) gen på inaktiva X-kromosomen.

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Fråga 133 x-kromosom inaktivering genetik epigenetik MeCP2 Rett syndrom XIST shRNA screening
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