É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:08Introduction
- 00:27CellChip preparation: Hydrophilization, coating, incubation
- 03:41Bioreactor Setup
- 07:43Incubation Setup
- 08:58Analysis of organotypic functions
Vi beskriver ett chip-baserad plattform för tredimensionella cellkulturer i mikro-bioreaktorer. Ett chip kan rymma upp till 10 miljoner. celler som kan odlas på exakt definierade villkor med avseende på strömning, syre spänning etc. i ett slutet, sterilt cirkulation slinga.
Tags
Chip-basedThree-dimensional Cell CulturePerfused Micro-bioreactorsChipNon-biodegradable PolymersBio-degradable PolymersDimensionsCubic Micro-containersRound RecessesScaffoldCellsNutrient SupplyGas SupplyBioreactor HousingClosed Steril Circulation LoopRoller PumpMedium ReservoirPerfusionSuperfusionMixed Operation ModeCell LinesPrimary CellsOrganotypic FunctionsHepatocellular Carcinoma Cell LinesLiver Specific Genes
