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Immunology and Infection

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JoVE Journal Immunology and Infection
Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
 

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

Article DOI: 10.3791/56421-v • 10:11 min • January 31st, 2018
January 31st, 2018 •
1, 1, 1, 2, 1
1Division of Infectious Diseases, Department of Medicine and Vancouver Costal Health Research Institute, University of British Columbia, 2Institute of Biomedical Technology, University of Tampere

Capítulos

Resumo
Automatic Translation

A novel technique for rapid antigen display on a bacterial surface is presented, which involves surface biotinylation followed by exposure to proteins of interest in fusion with monomeric avidin. Loading BCG with selected antigens successfully improves its immunogenicity, suggesting that surface decoration can replace traditional genetic approaches.

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Keywords: Mycobacterium Bovis BCG Surface Decoration Avidin-biotin System Exogenous Antigen Expression Recombinant Protein E. Coli BL21 Nickel NTA Column Protein Refolding Protein Purification
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