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非遗传表面修饰的素-生物素系统在牛分枝杆菌卡介苗中的外源抗原表达

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

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非遗传表面修饰的素-生物素系统在牛分枝杆菌卡介苗中的外源抗原表达

DOI:

10.3791/56421-v

•

10:11 min

•

January 31, 2018

•

Ting-Yu Angela Liao, Alice Lau, Joseph Sunil, Vesa Hytönen, Zakaria Hmama

¹Division of Infectious Diseases, Department of Medicine and Vancouver Costal Health Research Institute,University of British Columbia, ²Institute of Biomedical Technology,University of Tampere

Capítulos

00:05Título
01:00Monomeric Avidin Fusion Protein Expression, Purification, and Refolding
03:53Biotinylation of BCG Cell Surface
05:47Binding of Monomeric Adivin-fusion Protein to Biotinylated BCG Surface
07:06Results: Biotinylation with Avidin-OVA
09:13Conclusion

Summary

Tadução automática

提出了一种新的细菌表面快速抗原显示技术, 它涉及表面法, 其次是暴露于与单体素融合的感兴趣的蛋白质。用选定抗原载入卡介苗, 成功地提高了其免疫原性, 表明表面修饰可以取代传统的遗传方法。

Tags

Keywords: Mycobacterium Bovis BCG Surface Decoration Avidin-biotin System Exogenous Antigen Expression Recombinant Protein E. Coli BL21 Nickel NTA Column Protein Refolding Protein Purification

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