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Multiplex cytokin profilering af stimuleret mus Splenocytes ved hjælp af en Cytometric perle-baseret immunassay Platform
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
DOI:

11:00 min

November 09, 2017

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Capítulos

  • 00:05Título
  • 00:58Reagent and Standard Preparation
  • 02:57Assay Procedure
  • 06:23Flow Cytometer Set-up
  • 09:24Results: Quantification of Cytokines Produced by Mouse Splenocytes Cultured Under Stimulating Conditions
  • 10:15Conclusion

Summary

Tadução automática

Denne protokol beskriver kvantificering af flere cytokin mål samtidig i vævskultur analysere indsamlet fra stimuleret mus splenocytes ved hjælp af multiplex perle baseret immunassay platform og et flow Flowcytometret.

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