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利用彗星试验评价体外DNA 损伤
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JoVE Journal Pesquisa do Câncer
Evaluating In Vitro DNA Damage Using Comet Assay
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利用彗星试验评价体外DNA 损伤

DOI:
10.3791/56450-v

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10:43 min

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October 11, 2017

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, ,
1Neuro-Oncology Branch, Center for Cancer Research,National Cancer Institute, 2Basic Medical Science Department,Zunyi Medical College-Zhuhai Campus

Capítulos

  • 00:05Título
  • 00:46Preparing Comet Slides
  • 03:17Single Cell Electrophoresis
  • 05:43Staining Comet Slides
  • 06:25Image Acquisition and Analysis
  • 08:35Results: Combining Olaparib with Temozolomide Enhances DNA damage in Glioma Cells
  • 09:41Conclusion

Summary

Tadução automática

Tadução automática

彗星试验是一种有效的方法来检测 dna 损伤, 包括单和双 dna 断裂。我们描述了碱性和中性彗星检测肿瘤细胞 DNA 损伤, 以评估化疗的治疗效果。

Tags

Comet AssayDNA DamageGenotoxic AgentsCancer CellsSingle Cell ElectrophoresisAlkaline Comet AssayAgaroseCell CultureGlioma CellsChemotherapyLysis SolutionDNA UnwindingElectrophoresis

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