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Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping
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Medicina
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JoVE Journal Medicina
Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping

Electrophysiological Assessment of Murine Atria with High-Resolution Optical Mapping

DOI:
10.3791/56478-v

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08:19 min

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February 22, 2018

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, , , , ,
1Department of Bio-informational Pharmacology, Medical Research Institute,Tokyo Medical and Dental University, 2Department of Biofunctional Informatics,Tokyo Medical and Dental University

Capítulos

  • 00:04Título
  • 00:48Mouse Cannulation and Perfusion for Optical Mapping using Langendorff
  • 03:37Staining for Single Recording Membrane Voltage, Burst Pacing, and Extra-stimulus Pacing
  • 06:37Results: Conduction Patterns during Normal Sinus Rhythm and Atrial Tachyarrhythmia
  • 07:49Conclusion

Summary

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Tadução automática

This protocol describes the electrophysiological evaluation of murine atria utilizing an optical mapping system with a high temporal and spatial resolution, including dual recordings of the membrane voltage and Ca2+ transient under programmed stimulation through a specialized electrode catheter.

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Keywords: Electrophysiological AssessmentMurine AtriaHigh-resolution Optical MappingCardiac ElectrophysiologyAtrial ArrhythmiaLangendorff ApparatusPerfusion CircuitAnesthesiaHeart ExcisionTyrode’s SolutionPerfusion PressureVena CavaGlass ChamberVentricular CavityStimulation ElectrodeElectrocardiogram

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