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Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
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Biologia
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JoVE Journal Biologia
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

DOI:
10.3791/56482-v

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11:15 min

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January 04, 2018

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,
1Department of Convergence Medicine, University of Ulsan College of Medicine and Asan Institute for Life Sciences,Asan Medical Center, 2Synaptic Circuit Plasticity Laboratory, Department of Structure and Function of Neural Network,Korea Brain Research Institute

Capítulos

  • 00:05Título
  • 01:14Exosome Block Preparation
  • 03:38Sectioning, Staining, and Imaging of the Exosome
  • 04:27Immuno-staining of Sections and Imaging
  • 07:24Whole Mount for Immunostaining
  • 09:10Results: Immunostained Exosome Sections Provide Insight to Their Protein Makeup
  • 09:59Conclusion

Summary

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This protocol describes the various techniques necessary for transmission electron microscopy including negative staining, ultrathin sectioning for detailed structure, and immuno-gold labelling to determine the positions of specific proteins in exosomes.

Tags

ExosomesTransmission Electron MicroscopySample PreparationImagingExtracellular VesiclesProtein LocalizationUltracentrifugationFixationDehydrationEmbeddingLow Viscosity EmbeddingDisease DiagnosisMicrocellular Incubation

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