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人体滋养层模型研究母体肥胖炎症对胎盘自噬的调节作用
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JoVE Journal Medicina
A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
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人体滋养层模型研究母体肥胖炎症对胎盘自噬的调节作用

DOI:
10.3791/56484-v

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11:44 min

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September 27, 2017

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, ,
1Knight Cardiovascular Institute,Oregon Health and Science University, 2Department of Obstetrics and Gynecology,Oregon Health and Science University

Capítulos

  • 00:05Título
  • 00:56Collection of Placental Tissue
  • 01:42Isolation of Trophoblasts from Vilous Tissue
  • 05:11Density Centrifugation
  • 06:29Plating Cells
  • 07:18Treating Cells with TNFα and Harvesting Cells and Total Protein
  • 09:10Results: TNFα Regulates the Expression of Rubicon in Trophoblasts from Lean Pregnancies with Female Fetuses
  • 10:20Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了一种用于人胎盘绒毛组织的取样方法, 其次是分离细胞原代细胞培养。滋养治疗 TNFα重述炎症在肥胖的宫内环境和促进发现的分子靶调控的炎症在胎盘孕产妇肥胖。

Tags

TrophoblastInflammationMaternal ObesityAutophagyPlacentaTNF-alphaTrophoblast IsolationDigestionCell Culture

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