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Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
JoVE Journal
Biologia
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JoVE Journal Biologia
Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
DOI:

07:28 min

November 24, 2017

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Capítulos

  • 00:05Título
  • 00:45Culturing, Growth, and Target-specific Staining of A. tumefaciens Strains
  • 02:21Imaging of A. tumefaciens Cells
  • 04:54Epifluorescence and Time-lapse Microscopy
  • 05:41Results: Cells Growth of Live Labeled Microbial Cells Imaged with Fluorescence Microscopy
  • 06:47Conclusion

Summary

Tadução automática

Understanding the function of essential processes in bacteria is challenging. Fluorescence microscopy with target-specific dyes can provide key insights into microbial cell growth and cell cycle progression. Here, Agrobacterium tumefaciens is used as a model bacterium to highlight methods for live cell imaging for characterization of essential processes.

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