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Serum and Plasma Copy Number Detection Using Real-time PCR
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Serum and Plasma Copy Number Detection Using Real-time PCR

Serum and Plasma Copy Number Detection Using Real-time PCR

DOI:
10.3791/56502-v

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09:21 min

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December 15, 2017

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, , , , ,
1Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Capítulos

  • 00:05Título
  • 00:55Collection of Serum or Plasma
  • 02:20Isolating Cell-free (cfDNA) from Serum or Plasma
  • 05:15Real-time PCR and Analysis of cfDNA
  • 07:36Results: Detection of Androgen Receptor (AR) Copy Number Variations in cfDNA of Metastatic Castration-resistant Prostate Cancer (CRPC) Patients
  • 08:25Conclusion

Summary

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This manuscript describes the copy number variation analysis performed in serum or plasma DNA using real-time PCR approach. This method is suitable for the prediction of drug resistance in castration resistant prostate cancer patients, but it could be informative also for other diseases.

Tags

SerumPlasmaCopy Number DetectionReal-time PCRCell-free DNAAndrogen ReceptorGene Copy Number VariationMetastatic Castration-resistant Prostate CancerBiomarkerLiquid BiopsyDigital PCR

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