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Ein Protokoll für einem Maus Cochleae für das Innenohr Tissue Engineering
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JoVE Journal Bioengenharia
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
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Ein Protokoll für einem Maus Cochleae für das Innenohr Tissue Engineering

DOI:
10.3791/56523-v

•

09:53 min

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January 01, 2018

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, , , ,
1Department of Otolaryngology,University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering,University of Oklahoma, 3Department of Plastic Surgery,University of Kansas Medical Center

Capítulos

  • 00:05Título
  • 01:05Temporal Bone Harvest and Cochlea Isolation
  • 03:07Cochlear Processing
  • 05:11Infusion of hWJCs into Decellularized Cochleae
  • 06:58Results: Cell Culture on Decellularized, Decalcified Mouse Cochleae
  • 08:18Conclusion

Summary

Tadução automática

Tadução automática

Das Ziel dieses Protokolls ist eine effektive Methode, um decellularize und Entkalken Maus Cochleae zur Nutzung als Gerüste für Tissue-engineering-Anwendungen zu demonstrieren.

Tags

DecellularizationDecalcificationMouse CochleaExtracellular MatrixStem Cell DifferentiationSensory EpitheliumTemporal BoneBony LabyrinthStapesOval WindowRound WindowPerilymph1% SDS SolutionPBSAntibiotic-antimycoticPenicillin-streptomycin

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