Journal
/
Neurociência
/
Bruker en Microfluidics enhet for mekanisk stimulering og høy oppløsning Imaging C. elegans
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Bruker en Microfluidics enhet for mekanisk stimulering og høy oppløsning Imaging C. elegans

DOI:
10.3791/56530-v

•

10:39 min

•

February 19, 2018

•

, , , , ,
1Department of Molecular and Cellular Physiology,Stanford University, 2Department of Mechanical Engineering,Stanford University, 3Department of Bioengineering,Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology,The Institute of Photonic Sciences (ICFO)

Capítulos

  • 00:05Título
  • 00:52Preparation of the Microscope
  • 02:02Preparing the Microfluidic Chip
  • 03:45Loading the Test Subject
  • 06:02Stimulation and Removal of the Test Subject
  • 08:13Results: Touch Receptor Neuron (TRN) Stimuli
  • 09:42Conclusion

Summary

Tadução automática

Tadução automática

Nye verktøy for mechanobiology forskning er nødvendig for å forstå hvordan mekanisk stress aktiverer biochemical pathways og utløser biologiske respons. Her presentere vi en ny metode for selektiv mekanisk stimulering av immobilisert dyr med en microfluidic-felle slik at høy-resolution tenkelig mobilnettet svar.

Tags

MicrofluidicsMechanical StimulationHigh-resolution ImagingC. ElegansMechanobiologySensory BiologyPressure ActuatorsGCaMPRFPFluorescence MicroscopyM9 BufferPeristaltic PumpPDMS

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/56530?article_type=v

Assaying β-amyloid Toxicity using a Transgenic C. elegans Model
check_url/pt/56530?article_type=v

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network
check_url/pt/56530?article_type=v

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism
check_url/pt/56530?article_type=v

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior
check_url/pt/56530?article_type=v

The NeuroStar TMS Device: Conducting the FDA Approved Protocol for Treatment of Depression
check_url/pt/56530?article_type=v

Høyoppløselige funksjonell magnetisk resonans imaging metoder for Human midbrain
check_url/pt/56530?article_type=v

Høy oppløsning Live-Imaging of Cell Behavior i utviklingsland Neuroepithelium
check_url/pt/56530?article_type=v

Automatisert Kvantifisering av Synaptic fluorescens i<em> C. elegans</em
check_url/pt/56530?article_type=v

In vivo Neuronal Kalsium Imaging i C. elegans
check_url/pt/56530?article_type=v

Høy oppløsning<em> I Vivo</em> Manuell Segmentering Protokoll for menneskelig hippocampus subfields Bruke 3T Magnetic Resonance Imaging

Read Article