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Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
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Capítulos
- 00:05Título
- 00:59Preparation of Mouse Bone Marrow Derived Macrophages (BMDMs) and BSA-fatty Acid Conjugate
- 04:31Saturated Fatty Acids Induce Cell Death of Mouse Bone Marrow Derived Macrophages
- 06:04Intracellular Ceramide Staining
- 07:03Results: Fatty Acid-induced Macrophage Cell Death and Ceramide Production
- 07:47Conclusion
We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide levels.
