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Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
JoVE Journal
Biologia
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JoVE Journal Biologia
Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
DOI:

09:04 min

February 23, 2018

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Capítulos

  • 00:05Título
  • 01:16Cell Culture Monitoring Acquisition
  • 04:17Cell Culture Data Analysis
  • 05:59Results: A Lens-free Technique for Simultaneously Tracking the Kinetics of Thousands of Cells
  • 07:47Conclusion

Summary

Tadução automática

Lens-free video microscopy enables us to monitor cell cultures directly inside the incubator. Here we describe the full protocol used to acquire and analyze a 2.7 day long acquisition of cultured HeLa cells, leading to a dataset of 2.2 x 106 measurements of individual cell morphology and 10584 cell cycle tracks.

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