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无透镜视频显微术在黏附细胞培养动态定量分析中的研究

JoVE Journal
Biologia

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JoVE Journal Biologia

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

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无透镜视频显微术在黏附细胞培养动态定量分析中的研究

DOI:

10.3791/56580-v

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09:04 min

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February 23, 2018

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Cedric Allier, Romaric Vincent, Fabrice Navarro, Mathilde Menneteau, Lamya Ghenim⁴, Xavier Gidrol, Thomas Bordy, Lionel Hervé, Olivier Cioni, Sabine Bardin, Michel Bornens, Yves Usson⁶, Sophie Morales

¹CEA, LETI, DTBS, LISA,Université Grenoble Alpes, ²CEA, LETI, DTBS, LBAM,Université Grenoble Alpes, ³CEA, INSERM, BIG,Université Grenoble Alpes, ⁴CNRS, FR CNRS 3425, ⁵CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport,PSL Research University, Institut Curie, ⁶TIMC-IMAG

Capítulos

00:05Título
01:16Cell Culture Monitoring Acquisition
04:17Cell Culture Data Analysis
05:59Results: A Lens-free Technique for Simultaneously Tracking the Kinetics of Thousands of Cells
07:47Conclusion

Summary

Tadução automática

无透镜的视频显微镜使我们能够在孵化器内直接监测细胞培养。在这里, 我们描述了用于获取和分析2.7 天长时间获取培养的 HeLa 细胞的完整协议, 导致一个数据集 2.2 x 10⁶测量单个细胞形态学和10584细胞周期轨道。

Tags

Lens-free Video Microscopy Adherent Cell Culture Label-free Time-lapse Acquisition Holographic Reconstruction Hela Cells Fibronectin Coating Incubator-compatible CMOS Image Sensor LED Illumination

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