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Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
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1Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg ‘Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung’,Institutsteil Würzburg, 2Lehrstuhl für Tissue Engineering und Regenerative Medizin,Universitätsklinikum Würzburg, 3Lehrstuhl für Pharmazeutische Technologie und Biopharmazie,Universität Würzburg, 4Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften,Universität Würzburg
Capítulos
- 00:04Título
- 00:46Expression and Purification of BMP2-K3Plk
- 05:46Covalent Coupling Technique of BMP2-K3Plk to Azide Functionalized Agarose Beads
- 07:04Measuring Alkaline Phosphatase (ALP) Expression to Prove the In Vitro Bioactivity of the Produced BMP2-K3Plk Before and After the Coupling Reaction
- 08:34Results: Evaluation of the Bioactivity of BMP2-K3Plk after Site-Directed Immobilization
- 09:54Conclusion
Biomaterials doped with Bone Morphogenetic Protein 2 (BMP2) have been used as a new therapeutic strategy to heal non-union bone fractures. To overcome side effects resulting from an uncontrollable release of the factor, we propose a new strategy to site-directly immobilize the factor, thus creating materials with improved osteogenic capabilities.
