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In Vitro SUMOylation analyse at studere SUMO E3 Ligase aktivitet
 
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In Vitro SUMOylation analyse at studere SUMO E3 Ligase aktivitet

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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I modsætning til ubiquitin ligases, er par E3 SUMO ligases blevet identificeret. Denne modificerede in vitro- SUMOylation protokol er købedygtig identificere roman SUMO E3 ligases ved en in vitro- rekonstitution assay.

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Molekylærbiologi sag 131 In vitro SUMOylation K-bZIP SUMO E3 ligase p53 posttranslationel modifikation Ubc9
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