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In Vitro SUMOylation Assay te studeren SUMO E3 Ligase activiteit
 
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In Vitro SUMOylation Assay te studeren SUMO E3 Ligase activiteit

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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In tegenstelling tot ubiquitin ligases, zijn paar E3 SUMO ligases geïdentificeerd. Deze gewijzigde in vitro SUMOylation protocol vermag roman SUMO E3 ligases identificeren door een reconstructie in vitro assay.

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Moleculaire biologie kwestie 131 In vitro SUMOylation K-bZIP SUMO E3 ligase p53 posttranslationele modificatie Ubc9
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