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大鼠视网膜仿生化学调节的神经递质谷氨酸的体外实验方法

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Methodology for Biomimetic Chemical Neuromodulation of Rat Retinas with the Neurotransmitter Glutamate In Vitro

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大鼠视网膜仿生化学调节的神经递质谷氨酸的体外实验方法

DOI:

10.3791/56645-v

•

12:56 min

•

December 19, 2017

•

Corey M. Rountree, John B. Troy, Laxman Saggere

¹Department of Mechanical and Industrial Engineering,University of Illinois at Chicago, ²Department of Biomedical Engineering,Northwestern University

Capítulos

00:05Título
01:16Wholemount Retinal Preparation
03:12MEA and Data Acquisition Setup
05:23Glutamate Stimulation Preparation
08:38Retinal Recording and Stimulus Program
10:30Results: Electrode Recordings of Neuronal Activity During Visual, Spontaneous, and Glutamate Injection Recordings
12:06Conclusion

Summary

Tadução automática

本议定书描述了一种新的方法, 研究一种形式的化学神经的 wholemount 大鼠视网膜体外与神经递质谷氨酸。化学神经是一种有前途的替代传统的电神经视网膜神经元治疗不可逆转性失明引起的感光变性疾病。

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