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Establishment of an Extracellular Acidic pH Culture System
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Biologia
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JoVE Journal Biologia
Establishment of an Extracellular Acidic pH Culture System

Establishment of an Extracellular Acidic pH Culture System

DOI:
10.3791/56660-v

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09:41 min

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November 19, 2017

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,
1Innovative Technology Laboratories,Kyowa Hakko Kirin Co., Ltd, 2Laboratory for Systems Biology and Medicine, RCAST,The University of Tokyo

Capítulos

  • 00:05Título
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

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The acidic tumor microenvironment plays critical roles in tumor progression. To assess the effects of acidic extracellular pH on cancer cells in vitro, we established simple acidic culture systems.

Tags

Keywords: Extracellular Acidic PHTumor MicroenvironmentCancer ProgressionMalignancyAcidosisDMEMSodium BicarbonateL-glutaminePenicillin-streptomycinFetal Bovine SerumLactateHClPH MeasurementCell CulturePBSTrypsin

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