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胞外酸性 pH 培养体系的建立

JoVE Journal
Biologia

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JoVE Journal Biologia

Establishment of an Extracellular Acidic pH Culture System

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胞外酸性 pH 培养体系的建立

DOI:

10.3791/56660-v

•

09:41 min

•

November 19, 2017

•

Ayano Kondo, Tsuyoshi Osawa

¹Innovative Technology Laboratories,Kyowa Hakko Kirin Co., Ltd, ²Laboratory for Systems Biology and Medicine, RCAST,The University of Tokyo

Capítulos

00:05Título
00:57Preparation of Acidic Culture Medium
02:50Harvesting and Treating Cells
04:31RNA Isolation
06:30cDNA Synthesis and Analysis
08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
08:49Conclusion

Summary

Tadução automática

酸性肿瘤微环境在肿瘤进展中起重要作用。为了评估酸性细胞外 pH 对癌细胞的影响, 在体外,建立了简单的酸性培养体系。

Tags

Keywords: Extracellular Acidic PH Tumor Microenvironment Cancer Progression Malignancy Acidosis DMEM Sodium Bicarbonate L-glutamine Penicillin-streptomycin Fetal Bovine Serum Lactate HCl PH Measurement Cell Culture PBS Trypsin

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