Journal
/
Biologia
/
Etablering af en ekstracellulære sure pH kultur System
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Establishment of an Extracellular Acidic pH Culture System

Etablering af en ekstracellulære sure pH kultur System

DOI:
10.3791/56660-v

•

09:41 min

•

November 19, 2017

•

,
1Innovative Technology Laboratories,Kyowa Hakko Kirin Co., Ltd, 2Laboratory for Systems Biology and Medicine, RCAST,The University of Tokyo

Capítulos

  • 00:05Título
  • 00:57Preparation of Acidic Culture Medium
  • 02:50Harvesting and Treating Cells
  • 04:31RNA Isolation
  • 06:30cDNA Synthesis and Analysis
  • 08:01Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
  • 08:49Conclusion

Summary

Tadução automática

Tadução automática

Sure tumor mikromiljø spiller vigtige roller i tumor progression. For at vurdere virkningerne af sure ekstracellulære pH på kræft celler in vitro etableret vi enkle sure kultur systemer.

Tags

Extracellular Acidic PHTumor MicroenvironmentCancer ProgressionMalignancyAcidosisDMEMSodium BicarbonateL-glutaminePenicillin-streptomycinFetal Bovine SerumLactateHClPH MeasurementCell CulturePBSTrypsin

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/56660?article_type=v

Homemade Site Directed Mutagenesis of Whole Plasmids
check_url/pt/56660?article_type=v

Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
check_url/pt/56660?article_type=v

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications
check_url/pt/56660?article_type=v

The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
check_url/pt/56660?article_type=v

Mus embryonale udvikling i et serum-frit Hele Embryo Culture System
check_url/pt/56660?article_type=v

En<em> Ex vivo</em> Kultur System til Studieinformationen Thyroid Development
check_url/pt/56660?article_type=v

Udvikling af en Insert Co-kultur System af to Cellular typer i fravær af celle-celle-Kontakt
check_url/pt/56660?article_type=v

Etablering af en klonal kultur af encellede de tråddannende alger
check_url/pt/56660?article_type=v

Måling af sammenløbet af iPSC'er ved hjælp af et automatiseret billedbehandlingssystem.
check_url/pt/56660?article_type=v

Et tredobbelt kulturcellesystem, der modellerer den menneskelige blod-hjerne-barriere

Read Article