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Biologia do Desenvolvimento

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雌性生殖道交配后精子的收集及对小鼠精子液化的测定

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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

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雌性生殖道交配后精子的收集及对小鼠精子液化的测定

DOI:

10.3791/56670-v

•

12:06 min

•

November 18, 2017

•

Shuai Li, Wipawee Winuthayanon

¹School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine,Washington State University

Capítulos

00:00Título
02:02Mice, Mating, and Assessing Copulatory Plug
03:25Preparation Before Tissue Collection
04:40Tissue Collection
07:11Measurement of Semen Liquefaction from the Uterine Content Collection
09:25Resultados
10:02Example of Downstream Application: Video Imaging of Sperm Motility
11:13Conclusion

Summary

Tadução automática

精子的液化需要从精凝胶中解放出来。本研究提供了从雌性生殖道 post-coitus 中收集精子, 以及测定精子液化时间的程序。

Tags

Post-mating Semen Semen Liquefaction Mice Female Reproductive Tract Semen Collection Sperm Motility Prostate Specific Antigen (PSA)Infertility Vaginal Plug Leibovitz’s L-15 Media Fetal Bovine Serum

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