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宫内电穿孔诱导小鼠大脑皮质中氧合酶的重组

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation

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宫内电穿孔诱导小鼠大脑皮质中氧合酶的重组

DOI:

10.3791/56675-v

•

10:29 min

•

November 17, 2017

•

Katherine M. Bland*¹, Zachary O. Casey*¹, Christopher J. Handwerk*¹, Z. Logan Holley, George S. Vidal

¹Department of Biology,James Madison University

Capítulos

00:05Título
01:05Preparation of Pulled Pipettes
02:29In Utero Electroporation
06:54Brain Removal for Histological Preparation
08:06Results: Expression of GFP After Electroporation
09:18Conclusion

Summary

Tadução automática

在稀疏的细胞群中, 可以通过诱导功能的丧失或增益来研究脑内基因的细胞自主功能。在这里, 我们描述在子宫内电穿孔, 以提供重组到稀疏的人群发育皮层神经元与 floxed 基因, 导致功能丧失在体内。

Tags

Cre-lox Recombination Mouse Cerebral Cortex In Utero Electroporation Genetic Mosaic Neurobiology Layer 2-3 Cortical Neurons Pipette Preparation Embryonic Day 15.5 Anesthesia Mouse Surgery

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