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用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

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用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

DOI:

10.3791/56688-v

•

08:55 min

•

December 14, 2017

•

Genta Ito, Taisuke Tomita²

¹Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, ²Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Capítulos

00:05Título
00:53Collecting Cell Lysates
02:34SDS-PAGE and Gel Transfer
04:41Immunoblotting
06:22Results: P-tag Gel Analysis
07:50Conclusion

Summary

Tadução automática

本研究介绍了一种简单的方法检测内源性水平的 Rab10 磷酸化富亮氨酸重复激酶2。

Tags

Keywords: Rab10 Phosphorylation LRRK2 Activity SDS-PAGE Phosphate-binding Tag Parkinson’s Research HEK293 Cells Cell Lysis Bradford Assay Sample Preparation SDS-PAGE Gel Manganese Chloride

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