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Biologia
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Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
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Biologia
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JoVE Journal Biologia
Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

DOI:
10.3791/56702-v

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11:03 min

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January 22, 2018

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, , , , ,
1Clinical Neuroscience, Section for Ophtalmology and Vision,Karolinska Institutet, 2Clinical Sciences, Intervention and Technology, Division of Obstetrics and Gynecology,Karolinska Institutet

Capítulos

  • 00:05Título
  • 01:12Subretinal Injection of Sodium Iodate into a Large-eyed Animal Model
  • 04:36Subretinal Transplantation of hESC-RPE Cells in Treated Animals
  • 07:35In Vivo Retinal and Subretinal Imaging
  • 08:47Results: Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells
  • 10:22Conclusion

Summary

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Retinal pigment epithelial cells could serve as a cell-replacement therapy for the advanced form of dry age-related macular degeneration. This protocol describes the generation of a large-eyed model of geographic atrophy and the subretinal transplantation of human embryonic stem cell-derived retinal pigment epithelial cells into this model of disease.

Tags

Subretinal TransplantationHuman Embryonic Stem CellRetinal Pigment Epithelial CellsLarge-eyed Animal ModelGeographic AtrophyRegenerative MedicineAge-related Macular DegenerationOptical Coherence TomographyMicrosurgeryPars Plana TechniqueVitrectomySubretinal Injection

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