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一种实时3D 单粒子跟踪协议

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

A Protocol for Real-time 3D Single Particle Tracking

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一种实时3D 单粒子跟踪协议

DOI:

10.3791/56711-v

•

10:16 min

•

January 03, 2018

•

Shangguo Hou, Kevin Welsher

¹Department of Chemistry,Duke University

Capítulos

00:05Título
00:31Overview of the Apparatus
01:44Preparing the Samples
02:21Optimizing the Tracking Parameters
07:12Real-time Tracking of Freely Diffusing Nanoparticles
08:52Results: Recreating the Path of A Freely Diffusing Nanoparticle
09:37Conclusion

Summary

Tadução automática

本协议详细介绍了一种 real-time 3D 单粒子跟踪显微镜的构造和操作, 能够跟踪高扩散速度和低光子计数率的纳米荧光探针。

Tags

Keywords: 3D Single Particle Tracking Real-time Microscopy Virology 488 Nm Laser Electro-optic Deflectors Tunable Acoustic Gradient Lens Avalanche Photodiode FPGA Fluorescent Beads Phosphate Buffered Saline Water Nano Positioner Photo Detector Focal Plane Raster Scan

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