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Imunologia e Infecção

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荧光寿命成像显微镜观察细胞内圈套的贩运

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

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荧光寿命成像显微镜观察细胞内圈套的贩运

DOI:

10.3791/56745-v

•

08:55 min

•

December 29, 2017

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Daniëlle R. J. Verboogen, Maksim V. Baranov, Martin ter Beest, Geert van den Bogaart

¹Department of Tumor Immunology,Radboud University Medical Center

Capítulos

00:05Título
00:37Preparation of the Microscopy Samples
01:49Recording of the FLIM Data
03:34Conversion of the Photon Recordings to FLIM Images
04:19Fitting of Fluorescence Lifetime Histograms for Whole-cell FLIM Analysis
06:03Results: Syntaxin 4 and VAMP3 Interaction in Live Cell Visualized by FRET-FLIM
07:43Conclusion

Summary

Tadução automática

该协议描述了一种新的方法, 允许定量可视化的复杂形成的圈套蛋白, 基于 rster 共振能量转移, 和荧光寿命成像显微镜。

Tags

Keywords: SNARE FLIM Membrane Trafficking HeLa Cells Syntaxin-4 VAMP3 Syntaxin-3 MCitrine MCherry Fluorescence Lifetime Imaging Microscopy Confocal Microscopy Instrument Response Function

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