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Biologia do Desenvolvimento
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Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

DOI:
10.3791/56750-v

•

08:07 min

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January 06, 2018

•

, , ,
1Maine Medical Center Research Institute

Capítulos

  • 00:05Título
  • 00:41Collection Tube Preparation and Bone Harvest
  • 02:52Total Bone Marrow Cell (BMC) and Split BMC Population Plating and Culture
  • 04:45Bone Marrow Stem Cell (BMSC) Differentiation
  • 06:07Results: Representative BMSC Differentiation
  • 07:27Conclusion

Summary

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In this article, we present methods to isolate and differentiate bone marrow stromal cells and hematopoietic stem cells from mouse long bones. Two different protocols are presented yielding different cell populations suitable for expansion and differentiation into osteoblasts, adipocytes, and osteoclasts.

Tags

Bone Marrow Stromal CellsBMSCsBone And Adipocyte Progenitor CellsBone HarvestBone Marrow IsolationCell CultureCell DifferentiationOsteoclast ProgenitorsMouseVisual Demonstration

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