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小鼠骨髓基质细胞与骨破骨祖的分离、培养及分化
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
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小鼠骨髓基质细胞与骨破骨祖的分离、培养及分化

DOI:
10.3791/56750-v

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08:07 min

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January 06, 2018

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, , ,
1Maine Medical Center Research Institute

Capítulos

  • 00:05Título
  • 00:41Collection Tube Preparation and Bone Harvest
  • 02:52Total Bone Marrow Cell (BMC) and Split BMC Population Plating and Culture
  • 04:45Bone Marrow Stem Cell (BMSC) Differentiation
  • 06:07Results: Representative BMSC Differentiation
  • 07:27Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了从小鼠长骨分离分化骨髓基质细胞和造血干细胞的方法。两种不同的协议, 产生不同的细胞群体, 适合扩展和分化成成骨细胞, 脂肪和破骨。

Tags

Keywords: Bone Marrow Stromal CellsBMSCsBone And Adipocyte Progenitor CellsBone HarvestBone Marrow IsolationCell CultureCell DifferentiationOsteoclast ProgenitorsMouseVisual Demonstration

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