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肺定植法在肺部组织循环肿瘤细胞可视化中的建立
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The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
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肺定植法在肺部组织循环肿瘤细胞可视化中的建立

DOI:
10.3791/56761-v

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07:39 min

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June 16, 2018

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, , , , , ,
1The Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University, 3Trauma Office,Children’s National Health System

Capítulos

  • 00:04Título
  • 00:46Tumor Cell Recovery
  • 02:01Fluorescent Labeling and Lung Colonization
  • 04:17Confocal Microscopic Imaging
  • 05:20Results: Representative Lung Colonization Analyses
  • 06:44Conclusion

Summary

Tadução automática

Tadução automática

需要一个动物模型来破译循环肿瘤细胞 (CTCs) 在肿瘤转移过程中促进肺定植的作用。在这里, 我们建立并成功地进行了体内检测, 专门测试了高分子纤维连接蛋白 (polyFN) 组装对 CTCs 肺定植的要求。

Tags

Lung ColonizationCirculating Tumor CellsCancer MetastasisCell VisualizationCell CultureTrypsin-EDTACell SuspensionCentrifugationCFSETail Vein InjectionC57BL/6 Mouse

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